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![]() | Immunoblotting Whole cell extract of human HepG2 was separated on SDS-PAGE and probed with Rabbit Anti-EDEM2 (C-terminal) (Cat. No. E1782). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. Antibody 0.2 μg/mL 2. Antibody 0.5 μg/mL 3. Antibody 0.5 μg/mL + EDEM2 immunizing peptide (human, 558-572) |
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E1782
Sigma
Anti-EDEM2 (C-terminal) antibody produced in rabbit
~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution
| Synonym: | Anti-ER degradation enhancer, mannosidase alpha-like 2 |
Description
| Immunogen | synthetic peptide corresponding to amino acids 558-572 of human EDEM2, conjugated to KLH. The corresponding sequence is identical in mouse and rat EDEM2. |
| Physical form | Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide. |
Properties
| antibody form | affinity isolated antibody |
| clone | (polyclonal) |
| form | buffered aqueous solution |
| mol wt | antigen mol wt ~70 kDa |
| species reactivity | mouse, human, rat |
| concentration | ~1.0 mg/mL |
| application(s) | immunoblotting: 0.5-1.0 μg/mL using whole extracts of human HepG2 and rat NRK cells. |
| indirect immunofluorescence: suitable | |
| Gene Information | human ... EDEM2(55741) |
| rat ... Edem2(296304) | |
| mouse ... Edem2(108687) | |
| shipped in | dry ice |
| storage temp. | −20°C |







